Validation of multiplex PCR for detection and differentiation of Salmonellas
نویسندگان
چکیده
Methods The PCR-based detection technique for five Salmonella species was tested under O.I.E. requirements, to determine specificity, sensitivity, and repeatability. Primers were calculated by OLIGO software and produced with HPLC purification. Amplification was conducted by a T3000 Biometra PCR-machine. Testing was performed on a panel of five basic agents: Salmonella enterica ser. Enteritidis, Salmonella ser. Typhimurium, Salmonella ser. Typhi, Salmonella ser. Dublin, Salmonella ser. Gallinarum-Pullorum
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